Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs

The canine distemper virus (CDV), which infects dogs and a broad range of animal species, remains serious concern in Türkiye across the world. current study shows that CDV can be detected isolated rapidly specifically naturally infected dogs. Whole blood, nasal swab, ocular rectal urine samples from 50 stray were used (n = 250). presence genome was confirmed 105 (42%) using one-Step real-time RT-PCR. In total, 39 diagnosed with infection based on detection cytopathic effects MDCK, verified by fluorescent antibody technique. A total 12 RT-PCR negative samples, consisting 4 swabs 8 found to positive isolation. Blood, swab (P<0.01, r 1), 0.844), 0.697) all showed correlations tests for viral CPE levels high 37 (31.62%), medium 26 (22.23%) low 54 (46.15%) 117 (46.8%) growth cell culture. highest FAT samples. conclusion, one-step method proved very sensitive rapid reliable detection. Besides, non-modified MDCK isolate